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Mouse Genetic Monitoring Report
Rat Genetic Monitoring Report

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[SLC] 사육환경 설명서

Author
관리자
Date
2022-03-31 02:47
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89
  1. Production Style
    : For production of the uniform laboratory animal which maintained characteristics, maintenance and production of the strain is performed by strict production system based on the production control standard.
    1) Outbred: Maintenance and production by rotation breeding scheme
    2) Inbred

       2-1) Maintained colony: Strain maintenance and production of seed animals by the brother-sister mating
       2-2) Multiplication colony: Multiplication of seed animals used to Production colony and Hybrid(F1) production colony by the brother-sister mating in the maintained colony
       2-3) Production colony: Animal production by random mating in multiplication colony
    3) Hybrid: Hybrid (F1) production colony by mating between females and males of different inbred strain

  2. Breeding Environment
    : Animals are bred and cared in the following environment
    1) Room temperature: Mouse・Rat・Hamster・Mongolian gerbil - 23~25°C, Guinea pig - 21~25°C
    2) Humidity: 45~65%
    3) Ventilation frequency
    : All fresh air 10~15times/hour

    4) Differential pressure
    : 50~200Pa(5~20mmH2O)

    5) Illuminance
    : 150~300Lux (80cm above the floor)

    6) Lighting cycle
    : Light (8:00~20:00)、Dark (20:00~8:00)

    7) Air
    : Sterilized by 3-stage filters; front,middle,final (HEPA)

    8) Aeration
    : Pan-type air conditioning system is used as flow system


  3. Breeding Environmental Monitoring
    : Examination of drinking water, and performance tests of autoclave and ethylene oxide gas sterilizers are periodically performed.

  4. Animal Behavioral Monitoring
    : Production efficiency of each production colony is monitored and recorded in weekly report.
      Breeding technical person carefully observes animal and detects early abnormal animal.

  5. Feeds
    : Animals are given following diet;
      - Autoclaved diets (Nosan Corporation, PMI Inc.) 
      - Irradiated diets (PMI Inc., Funabashi Farm Co.,Ltd.)

  6. Microbiological Monitoring
    : 별도 게시글 참조

  7. Genetic Monitoring

  8. : Tested only mouse Inbred Strains
    1) Test items: Biochemical, immunogenetic and molecular-genetic markers.
    2) Test animals: Animals from each foundation stock colony and multiplication colony
    3) Test frequencies: At least once a year for each test item in each strain.

  9. Physiological Parameter Tests
    1) Body weight measurement
    2) Organ weight measurement- Test items:brain・heart・lung・liver・spleen・kidney・adrenal・testis・ovary・thymus・pituitary gland
    3) Hematological tests- Test items:RBC・Ht・Hb・WBC・PLT・MCV・MCH・MCHC
    4) Blood biochemical tests- Test items:AST・ALT・ALP・TG・ALB・TP・A/G・GLU・PL・T-BIL・BUN・T-CHO・Ca・IP・CRE・Na・K・Cl